22.06.15
KhoomKev ntes lub teeb pom kev tsis pom kev yog qhov nyuaj kom ua tiav cov duab zoo sib xws hauv ntau daim ntawv thov duab.Digital Scanned Light Sheet Microscopy (DSLM) muaj txoj hauv kev zoo los txo cov teeb pom kev tsis pom kev los ntawm synchronizing illumination nrog 'rolling shutter' ntawm niaj hnub CMOS koob yees duab.Txawm li cas los xij, qhov tseeb synchronization yuav tsum tau ua tiav kev tswj hwm ntawm lub koob yees duab dov shutter - ib qho tshwj xeeb muab los ntawm Tucsen koob yees duab nrog Rolling Shutter Control Mode.
Rolling Shutter yog dab tsi?
Lub Shutter yog ib feem ntawm lub koob yees duab uas pib thiab nres qhov raug ntawm lub koob yees duab rau lub teeb.Yav tas los, cov koob yees duab kev tshawb fawb tau siv cov tshuab kaw, qhib kom nthuav tawm cov duab, thiab kaw kom xaus qhov raug.Mechanical shutters tau qeeb thiab raug teeb meem kev ntseeg siab nrog kev siv txuas ntxiv.Tam sim no, cov koob yees duab kev tshawb fawb siv hluav taws xob kaw, uas tau nrawm dua, yooj yim dua, thiab ntau yam ntxiv.
Rolling shutter koob yees duab pib lawv cov khoom tau los ntawm sab saum toj ntawm lub sensor, thiab 'yob' kab los ntawm kab mus rau hauv qab ntawm lub sensor.Qhov kev yuav khoom no suav nrog peb txheej txheem: teeb liab pib dua, pom lub teeb, thiab nyeem ntawv.
Kev nthuav tawm ntawm txhua kab yog pib los ntawm kev rov pib dua txhua pixel lub teeb liab tau txais.Tom qab lub sijhawm raug xaiv tau dhau mus rau cov kab saum toj kawg nkaus, cov ntawv nyeem, cim qhov kawg ntawm qhov tau txais rolls hauv tib txoj kev.Qhov no tawm ntawm thaj tsam ntawm cov pixels nquag uas cheb los ntawm sab saum toj mus rau hauv qab ntawm lub koob yees duab, nrog nws qhov siab txiav txim siab los ntawm qhov ntev ntawm lub sijhawm raug.Nrog lub koob yees duab khiav ntawm qhov nrawm, qhov ncua sij hawm ib kab yog feem ntau ntawm 5 thiab 25 microseconds ib kab ntawm pixels, nyob ntawm qhov ceev ntawm lub koob yees duab.
Txhawm rau kom tau txais txiaj ntsig zoo ntawm cov txheej txheem kho qhov muag xav tau synchronization ntawm scanning ntawm illumination thiab lub koob yees duab dov shutter, feem ntau qhov ncua sij hawm no luv luv, txhais tau tias lub dov shutter ua haujlwm sai heev rau lwm yam khoom siv kom ruaj.Qhov no yog qhov Rolling Shutter Control Mode tuaj rau hauv.
Daim duab 1: Schematic daim duab ntawm dov shutter ua haujlwm
Yuav Ua Li Cas Rolling Shutter Control Hom Ua Haujlwm
Los ntawm onboard txawj ntse nyob rau hauv Tucsen koob yees duab, kev ua hauj lwm ntawm lub koob yees duab lub dov shutter yuav ua tau zoo-tuned rau synchronize nrog lwm hardware.Los ntawm kev ntxiv me me ncua sij hawm nruab nrab ntawm qhov pib dua thiab nyeem tawm ntawm txhua kab, lub sijhawm siv rau thaj chaw pixel nquag los cheb lub sensor tuaj yeem tswj tau kom tso cai rau qhov kev sib koom ua ke no.
Tsis tas li ntawd, qhov 'slit qhov siab' ntawm qhov chaw ua haujlwm scanned tuaj yeem raug kho kom zoo.Lub sij hawm raug ntev dua los yog ncua sij hawm kab luv luv ua rau qhov siab qhov siab loj dua.Nyob rau hauv rooj plaub ntawm DSLM, qhov no tuaj yeem siv los ua kom haum rau thaj tsam ntawm qhov pom ntawm tus qauv, tawm tsam qhov sib npaug ntawm kev nthuav tawm pixels rau lub sijhawm siab tshaj plaws rau kev ntes cov teeb liab zoo, thiab txo qis tawm ntawm lub teeb pom kev.
Daim duab2: Sab laug: Schematic ntawm dov shutter ua haujlwm ntawm lub koob yees duab nrawm.Txoj Cai: Schematic qhia dov shutter ceev nrog Rolling Shutter Control hom ntxiv ncua ncua ntawm txhua kab kom tso cai synchronization nrog lwm yam khoom siv.
Nrog rau qhov kev xaiv ncua sij hawm no, tam sim no muaj peb qhov tseem ceeb hloov pauv kom nkag siab tias txiav txim siab qhov kev ua haujlwm ntawm dov shutter, qhia qhov siab ntawm thaj tsam ntawm 'active' pixels, thiab ceev npaum li cas nws hla lub sensor.
Kab Sijhawm: Qhov no yog lub sij hawm uas lub sensor siv los nyeem ib kab thiab txav mus rau lwm qhov.Nws txiav txim siab haiv neeg 'ceev' ??ntawm lub koob yees duab sensor, thiab tuaj yeem teev nyob rau hauv lub koob yees duab software, los yog kwv yees rau thaj tsam ntawm kev txaus siab (ROI) thiab lub koob yees duab hom los ntawm:
Qhov twg 'Maximum Lub Koob Yees Duab Ncej Tus Nqi' yog hais txog tus ncej tus nqi thaum tsis txwv los ntawm ob lub sijhawm ntev los yog qhov cuam tshuam sab nraud.
Lub sij hawm nphav raug:Qhov no txiav txim siab ntev npaum li cas txhua pixel kab ua haujlwm rau, yog li txiav txim siab qhov siab ntawm thaj chaw ua haujlwm rau lub sijhawm muab kab thiab ncua sijhawm.
Kab ncua sij hawm:Qhov no yog qhov ncua sij hawm ntau npaum li cas ntxiv los ntawm Rolling Shutter Control Mode.Rolling Shutter Control Hom tso cai ncua ntxivnyob rau hauv integer multiples ntawm kab lub sij hawm.Piv txwv li, yog hais tias lub sij hawm kab rau lub koob yees duab yog 10 microseconds, ntxiv ncua ib kab ntawm 1, 2,…mus txog 8,928, tuaj yeem muab ntxiv, qhia tus lej ntawm qhov sib npaug ntawm 10 microseconds.
Tsis tas li ntawd tseem ceeb yog qhov siab ntawm thaj tsam ntawm kev txaus siab (ROI) siv, vim qhov no yuav txiav txim siab cov kab uas qhov chaw ua haujlwm yuav tsum cheb ua ntej rov pib dua.
Rolling Shutter Control Hom Synchronization hom
Muaj ob hom kev ua haujlwm rau Rolling Shutter Control Hom, nyob ntawm seb qhov kev hloov pauv tseem ceeb dua los tswj.
In Kab Sijhawm Delay hom, koj tuaj yeem teem sijhawm ncua raws li teev saum toj no.Software tuaj yeem qhia tau tias, rau koj lub sijhawm raug teeb tsa, qhov tshwm sim qhov siab qhov siab yuav ua li cas - qhov siab ntawm cov pixels nyob rau hauv lub kaw lus dov.
In Active Pixel / Slit Qhov siabhom, koj tuaj yeem teeb tsa tus naj npawb ntawm kab ntawm lub sensor koj xav kom ua haujlwm raws li lub kaw lus dov cheb.Lub sij hawm raug koj tau teev tseg yuav raug siv los xam qhov yuav tsum tau Kab Sijhawm Ncua kom xa qhov siab qhov siab txiav.
Teeb tsa Rolling Shutter Control hom hauv Software
Kev Ua Haujlwm Hom (Txheej xwm) Tswj
?
Daim duab 3: Piv txwv interface rau kev tswj Rolling Shutter Control hom los ntawm Tucsen Mosaic software.Txhua txoj kev xaiv?muaj nyob ntawm Micro-Manager thiab SDK.
Peb Qhov xwm txheej (kev ua haujlwm hom) muaj:Off, Kab ncua sij hawm, Slit Qhov siab.
? Thaum teem rauTawm, lub sensor coj li qub uas tsis muaj kev ncua ntxiv ntxiv.
? Thaum teem rauKab ncua sij hawmhom, koj tuaj yeem hais qhia txog Kab Sijhawm ncua sijhawm nyob rau hauv units ntawm kab sijhawm, raws li tau piav qhia saum toj no.
Daim duab 4: Line Time Delay software xaiv.Piv txwvinterface los ntawm Tucsen Mosaic software.Txhua qhov kev xaiv muaj nyob ntawm Micro-Manager thiab SDK.
Tus naj npawb ntawm kab lub sij hawm voj voog uas tuaj yeem ntxiv rau qhov kev ncua sij hawm sib txawv ntawm lub koob yees duab mus rau lub koob yees duab.Lub sijhawm kab tshiab ntawm lub koob yees duab, tom qab ncua ntxiv, yog:
Line Interval Time = Kab Sijhawm(sensor)+ (Lub sijhawm kab(sensor)× Kab ncua sij hawm)
Tus nqi parameter ntawmRolling Ceevyog sib npaugLub Sijhawm Kab Ntsig.
Lub sijhawm nyeem ntawv tag nrho ntawm daim duab yog tom qab ntawd:
Rua edTawm Sijhawm(duab)= Kab ncua sij hawm×Nkab.
Nkabyog tag nrho cov kab ntawm cov duab pixels hauv cheeb tsam txaus siab.Tus ncej tus nqi thaum imaging nyob rau hauv hom no nyob ntawm tus naj npawb ntawm cov kab uas yuav tsum tau imitation thiab kab lub sij hawm voj voog:
Ncej tus nqi = 1/(Readout Time(duab)+ Lub Sijhawm Tawm)
?Thaum teem rauSlit Qhov siab?mode, koj tuaj yeem teeb tsaqhov loj ntawm qhov chaw ua haujlwm scanned, muab los ntawm tnws tus lej pixel kab nruab nrab ntawm lub teeb liab "rov qab" thiab "readout" teeb liab.Qhov ntau ntawm Slit Height yog 1 ~ 2048, hauv pixel units.Txhawm rau hloov qhov no mus rau qhov loj me, muab tus nqi no los ntawm pixel loj los ntawm daim ntawv qhia lub koob yees duab.
Daim duab 5: Slit Height hom tswj kev xaiv.Piv txwvinterface los ntawm Tucsen Mosaic software.Txhua qhov kev xaiv muaj nyob ntawm Micro-Manager thiab SDK.
Lub software yuav cia li xam qhov yuav tsum tau Kab Sijhawm ncua sij hawm thiab Line Interval Time, tus qauv yog raws li nram no:
Kab Sijhawm ncua = Lub Sijhawm Tawm(kab)/ Slit Qhov siab(kab)
Thaum nyob rau hauv High Speed ??Mode (tau txais hom koob yees duab), Slit Height ntau tsuas tuaj yeem raug teeb tsa rau tus lej txawm tias hauv hom ntawd cov kab tau nyeem ob-los-ob.Cov tsis nyob rau hauv High Speed ??Mode yog xam raws li hauv qab no.
Kab Sijhawm ncua = Lub Sijhawm Tawm(kab)/ ? Qhov siab(kab)
Qhov siab = (Lub sij hawm nphav raug(kab)÷ Kab ncua sij hawm) × 2
Scan Direction Controls
Muaj peb txoj kev xaiv rau kev taw qhia ntawm dov shutter:
Dtus kheej： Cov kev taw qhia hauv qab scan yog lub neej ntawd scan kev taw qhia rau sCMOS koob yees duab.Lub dov shutter pib los ntawm thawj kab nyob rau sab saum toj ntawm Sensor thiab scans mus rau kab kawg nyob rau hauv qab.Txhua tus ncej tom ntej yuav tau pib nrog thawj kab nyob rau sab saum toj.
Daim duab 6: Downward Scan hom Schematic
Up:Nyob rau hauv lub upward scan hom, lub dov shutter pib los ntawm kab hauv qab thiab scans mus rau sab saum toj kab ntawm thawj kab.Txhua tus ncej tom ntej yuav tau pib los ntawm kab hauv qab.Txawm hais tias qhov kev txiav txim ntawm cov ntaub ntawv ntes ntawm lub koob yees duab tam sim no thim rov qab, cov duab uas tau muab xa mus rau software tseem yuav pom qhov qub kev taw qhia, piv txwv li cov duab yuav tsis raug flipped vertically txheeb ze rau hauv hom scan.
Daim duab 7: Upward Scan hom Schematic
Down-Up Cycle：Thaum luam theej tawm ua ke thiab nqis, lub kaw lus dov pib los ntawm thawj kab nyob rau sab saum toj thiab nqis mus rau kab kawg hauv qab.Rau lub thav duab tom ntej, lub dov shutter pib ntawm kab hauv qab thiab scans mus rau kab saum toj kawg nkaus, thiab lwm yam.Txoj kev taw qhia ntawm cov duab tau txais hauv hom no yog tib yam li qhov kev taw qhia kev ntsuas qis qis.
Daim duab 8: Down-Up Cycle Scan hom Schematic
? Readout Direction Pib dua
Txoj haujlwm no tsuas yog muaj nyob rau hauv Down-Up Cycle hom.
Lub neej ntawd teeb tsa rau qhov ntsuas no yog "Yog", uas ua kom ntseeg tau tias thawj tus ncej ntawm txhua qhov kev yuav khoom tshiab yuav pib ntawm kab saum toj kawg nkaus thiab luam theej tawm.
Thaum qhov kev ntsuas no tau teem rau "Tsis yog", thawj tus ncej ntawm txhua qhov kev yuav khoom tshiab yuav pib ntawm txoj haujlwm ntawm tus ncej kawg hauv qhov ua ntu zus dhau los.Yog tias tus ncej kawg xaus rau hauv kab hauv qab, thawj tus ncej ntawm kev yuav khoom tom ntej yuav pib ntawm kab hauv qab thiab nthuav tawm.
